Bioinformatic analysis of candidate Zif268-regulated proteasome genes. The 1 kb
upstream regions from the putative Zif268-regulated proteasome genes were compared
with the equivalent regions from 20 randomly selected genes (see Materials and
Methods and supplemental Table 2, available at www.jneurosci.org as supplemental
material). The numbers of genes are shown in parentheses.
a, The number of predicted EREs is significantly
greater in the proteasome genes. ***p < 0.001 versus randomly
selected genes (Mann–Whitney U test).
b, Frequency distribution of EREs in proteasome
genes (top) and randomly selected genes (bottom). EREs are concentrated immediately
upstream of the TSS in the proteasome genes. c, The
number of predicted NFκB binding sites is significantly greater in the
proteasome genes. *p < 0.05 versus randomly selected genes
(Mann–Whitney U test). d, The
number of predicted NFκB/Sp1/ERE modules is significantly greater in the
proteasome genes. For the randomly selected genes, no NFκB/Sp1/ERE modules
were detected. **p < 0.001 versus 0 (Wilcoxon test). Results are
shown as mean ± SEM.