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. 2006 Feb 1;26(5):1624–1634. doi: 10.1523/JNEUROSCI.4199-05.2006

Figure 3.

Figure 3.

Validation of psmb9, psme2, Tap1, and SGK regulation by Zif268 in PC12 cells. Semiquantitative RT-PCR was used to determine the relative expression levels of the genes. a, Representative RT-PCR product band intensities in pZif268- and ptrZif268-transfected PC12 cells. Note the stronger band intensities for Tap1, psmb9, SGK, and psme2 in the cells transfected with the truncated Zif268 expression vector ptrZif268. b, Relative amounts of target cDNA were determined (see Materials and Methods) 48 h after transfection. Results from five independent experiments are shown from ptrZif268 (□) and pZif268 (▪)-transfected cells (means ± SEM). *p ≤ 0.05; **p < 0.01, expression levels lower in pZif268-transfected PC12 cells compared with ptrZif268-transfected cells (Mann–Whitney U test). c, Levels of psmb9 protein immunoreactivity (psmb9-ir) in pZif268- and ptrZif268-transfected PC12 cells. The results suggested a corresponding regulation of protein levels.