Table 1.

RT-PCR primer sequences used during this study

Gene targeta	Oligonucleotidesb				Gene/locus accession numberc	Amplicon size (bp)
	Sense		Antisense
	Exons	Sequence 5′–3′	Exons	Sequence 5′–3′
Gapdh	1	CCGCTGATGCCCCCATGTTTGTGAT	1	GGCATGTCAGATCCACAACGGATAC	24383	369
psme2	6–7	AAGTGCATTCTGGTAATCACATGGATC	10–11	GATGAAGCTCAGCATAGAAGGCTCTC	29614	304
Mouse psme2	6–7	AAGTGCATTCTGGTAATCACATGGATC	10–11	GATAAAGCTCAGCATAGAAGGCCCTC	19188	304
psmb9	1–2	AGTCCACACCGGGACAACCATCATG	5–6	CCAGAGTGATGGCGTCTGTGGTGAA	24967	497
SGK	3–4	AACCCCTCACCTCCTCCAAGTCCCTCTCA	8–9	AGGAGCGAGATACTCAGGCGTGCCACA	29517	585
Tap1	5–6	TGGACCATGAGTGTCTCGGGAATGC	9–10	CAGTTTCACCTACCTCTGTGTCATAGCCCT	24811	682

^aPrimers target rat cDNA template unless otherwise denoted.

^bWhere possible, at least one of the primer pairs were predicted to span an exon–intron boundary. The exon number for which a section of the primer sequence is predicted to target are denoted. For primers predicted to span exon–intron boundaries, the portions of the primer sequence that target distinct exons are characterized by alternating type style (roman or italic/underline).

^cThe Entrez Gene ID is provided. The exon–intron structure information and sequence template for primer design were also obtained from the ENSEMBL database (www.ensembl.org).