Figure 1.

The endocannabinoid system is expressed in postnatal neural progenitors. A, Immunofluorescence was performed to assess CB₁ and FAAH (red) expression in nestin-GFP-derived neurospheres. Scale bar, 20 μm. B, Immunofluorescence was performed with CB₁ or FAAH antibodies (red) in combination with nestin, RC2, vimentin, or phosphorylated vimentin (4A4) antibodies (green). In addition, triple labeling was performed with CB₁ (blue), nestin (green), and BrdU (red) antibodies. Scale bars, 20 μm. C, The presence of CB₁ receptor and FAAH mRNA was determined by RT-PCR in NPs, differentiated neurons (N), and differentiated astroglial cells (A).