Figure 2.

Characterization of postnatal progenitor proliferation and astroglial differentiation. A, Distribution of the phenotypic analysis with the indicated cell markers of proliferating (Ki67⁺) cells after 16 h of differentiation. Representative immunofluorescence pictures of NPs at different stages of differentiation using the indicated markers (n.d., not determined). Scale bar, 30 μm. B, CB₁ receptor distribution in the different cellular phenotypes (as above). Representative nestin⁺GFAP⁺ CB₁⁺ (filled arrow) and nestin⁻GFAP⁺ CB₁⁺ cells (open arrow) are shown. Scale bar, 30 μm. Quantification of CB₁ distribution compared with the proliferative effect induced by WIN-55,212-2 for each cell type. Significantly different from controls, *p < 0.01. C–F, Cannabinoid impact on NP differentiation. Quantification of the percentage of nestin⁺ (C), GFAP⁺ (D), β-tubulin III⁺ (E), and nestin⁺GFAP⁺ (F) cells versus total cell number at the indicated differentiation times in the presence of vehicle, WIN-55,212-2, or URB597 alone or with SR141716, and SR141716 alone. Results correspond to three independent experiments. Significant differences between cannabinoid-treated versus control cells are shown. *WIN-55,212-2, ^#URB597, p < 0.05; ^£WIN-55,212-2, ^§URB597, p < 0.01.