Figure 5.

Inhibition of LIMK prevents acute cocaine-induced increase in p-cofilin. Three weeks after discontinuing repeated saline or cocaine (see Fig. 1a), rats were injected into the nucleus accumbens with a peptide antagonist of LIMK fused to the membrane permeability domain of HIV-Tat (1 nmol; Tat-cof) or a scrambled fusion peptide control (1 nmol; Tat-sc), injected 30 min later with cocaine (30 mg/kg, i.p.) or saline, and killed 45 min later, and F-actin-enriched subfraction of accumbens was examined for various proteins. Data are shown as mean ± SEM, and the number of determinations is shown in each bar and were statistically evaluated using a two-tailed Student's t test: Sa/Coc, p-cofilin, t₍₈₎ = 2.91, p = 0.020; Sa/Coc, Arp3, t₍₇₎ = 2.34, p = 0.051; Coc/Sa, Arp3, t₍₈₎ = 2.63, p = 0.030. *p < 0.05 comparing Tat-cof with Tat-sc.