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. 2019 Aug 1;10(4):731–745. doi: 10.14336/AD.2018.1001

Figure 4. Gal-3 Knockdown can alleviate MCT related vascular hypertrophy through inhibition of EndoMT.

Figure 4.

Representative immunofluorescence images of vWF and αSMA in pulmonary arteries (PAs) tissue sections from Neg Con, MCT+ Neg Con, MCT+Gal3KD or MCT+Gal3OE Rats, Scale bar, 50 μm. (B and C) Immunofluorescence staining of Gal-3 colocalized with vWF and αSMA. Scale bar, 50 μm. (D) Immunoblot of lung tissue lysate of indicated treated rats and (E) densitometric quantification, n=5. Arrow indicates representative PAs. DAPI indicates 4′,6-diamidino-2-phenylindole; Gal-3, Galectin-3; EC, endothelial cell; EndoMT, endothelial-to-mesenchymal transition; Neg Con, Negative control; Gal3KD, Gal-3 knockdown; Gal3OE, Gal-3 overexpression; αSMA, α smooth muscle actin.