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. 2019 Jul 25;4(14):e126325. doi: 10.1172/jci.insight.126325

Figure 4. S100a4-Cre is expressed in CD45+ cells in the gonads.

Figure 4

(A) Representative images of IF staining of GFP and S100A4 in the ovaries of reporter controls and homozygous Ptch1 mutants (both express GFP driven by S100a4-Cre). CL, corpus luteum; F, follicle; AF, atretic follicle. Scale bars: 200 μm (upper panels) and 100 μm (lower panels). (B) Representative images of IF staining of GFP with vimentin or ACTA2 in the ovaries of reporter controls and homozygous Ptch1 mutants. Scale bars: 40 μm (upper panels) and 75 μm (lower panels). (C) Representative images of IF staining of GFP and IHC staining of CD45 on adjacent serial sections of ovaries from reporter controls and homozygous Ptch1 mutants. Scale bar: 100 μm. (D) The percentage of CD45-positive cells among GFP-positive cells in the ovaries of S100a4-Cre;mTmG reporter control mice analyzed by flow cytometry. The image represents results from 3 independent samples, each of which contained dispersed ovarian cells pooled from 4 females. (E) Representative images of Xgal signal in testes of Ptch1-Xgal mice, as well as S100A4 and GFP staining in the testis of S100a4-Cre;mTmG reporter control mice. Arrows point to cells positive for GFP alone (green), S100A4 alone (red), or for both GFP and S100A4 (white). Scale bars: 50 μm and 20 μm (bottom right). (F) Representative images of IF staining of GFP and CYP17A1 in testes of S100a4-Cre;mTmG reporter control mice. The right panel is a zoomed-in view of the same tissue and staining as the left panel. Scale bars: 50 μm (left image) and 20 μm (right image).