Figure 4. ATGL ablation rescues lipodystrophy and its associated insulin resistance in Bscl2^–/– mice.

(A–C) Percentage of fat mass assessed by NMR, and masses of gonadal white adipose tissue (gWAT) and subcutaneous WAT (sWAT) as normalized to body weight. (D and E) Representative images and H&E staining of gWAT and sWAT. Scale bar: 200 μm. (F) Representative Western blotting of mature adipose marker and lipid droplet protein (LDP). (G) Plasma insulin levels after 4-hour fast. For A–G, 10-week-old female Atgl^+/+Bscl2^+/+ (A^wB^w), Atgl^+/+Bscl2^–/– (A^wB^k), Atgl^+/–Bscl2^–/– (A^hB^k), Atgl^–/–Bscl2^–/– (A^kB^k), and Atgl^–/–Bscl2^+/+ (A^kB^w) littermates were used for all experiments (n = 5–10/group). Limited numbers of male A^kB^k mice were obtained. However, a similar extent of rescue of lipodystrophy was observed in male A^hB^k and A^kB^k mice. (H and I) Insulin tolerance test and area under the curve (AUC) in mice other than A^kB^w (male and female, n = 5–7/group). *P < 0.05; **P < 0.005 by 1-way ANOVA with Dunnett’s multiple-comparisons correction.