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. 2019 Jul 25;4(14):e124716. doi: 10.1172/jci.insight.124716

Figure 3. In damaged SnCs, but not in CDKI-induced SnCs, the p53/p16-independent upregulation of NKG2D ligands regulates the balance between NKG2D-mediated immune detection and clearance of SnCs versus SnC survival and persistence.

Figure 3

(A) Survival of cells senesced under different conditions, and cocultured with IL-2–preactivated PBMCs or NK cells treated with a NKG2D receptor-blocking antibody, or IgG control, prior to coculture. Survival was evaluated by LDH release after 16 hours (effector/target ratio, 1:5). Black squares below the bar plot indicate which cells were tested in ≥2 independent experiments per cell condition and coculture setting. Box plot length: 25% and 75% of data; centerline: median; whiskers: 25% – (or 75% +) 1.5 × IQR, dots: outliers; color bars, averages. (B) Survival of cells senesced under different conditions and cocultured with PBMCs. Survival in triplicate was scored as the number of fibroblasts after 10 days in coculture relative to fibroblast monoculture. Effector/target ratio, 10:1. (C) Schematic summary.