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. 2019 Jul 25;4(14):e124716. doi: 10.1172/jci.insight.124716

Figure 8. Damaged SnCs that persist suppress NKG2D-dependent immunosurveillance.

Figure 8

(A) NKG2D mRNA levels were measured by quantitative real-time PCR (qRT-PCR) in IL-2–activated NK cells maintained for 48 hours in media supplemented with conditioned media from the indicated cells (4 rounds of treatment). mRNA levels were normalized to GUS; mock indicates regular medium. (B) Expression of NKG2D measured by qRT-PCR in PBMCs after 10 days of direct coculture with PRE or SEN fibroblasts. Expression of NKG2D was undetectable in fibroblasts (data not shown). Box plot length: 25% and 75% of data, centerline: median, whiskers: 25% – (or 75% +) 1.5 × IQR; color bars: median. (C) Schematic summary.