(A) Schematic of protocols for establishing oxazolone-induced (Oxa) and urushiol-induced (Uru) ACD in mice. Control group received vehicle (acetone) treatment only. TEWL, transepidermal water loss. (B) Molecular structure of oxazolone and urushiol (15:1). (C) Representative photographs of neck skin from mice treated with vehicle, oxazolone, or urushiol. (D) Dermatitis subscores of mice treated with vehicle (Veh), Oxa, or Uru. (E) Total dermatitis scores, derived by summation of subscores derived from D. (F) Plasma IgE level determined by ELISA. (G) Changes of neck skin bifold thickness. (H) TEWL measured at the neck skin. Data in bar graphs are expressed as mean ± SEM. n = 6–7 mice/group. **P < 0.01, ##P < 0.01. One- or 2-way ANOVA, followed by Tukey’s post hoc test, was used for statistical analysis.