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. 2019 Jul 25;4(14):e125652. doi: 10.1172/jci.insight.125652

Figure 1. Characterization of human iPSC-derived EVs.

Figure 1

(A) Bar graph and (B) scatter plot indicate the level of calcein-FITC+ iPSC-derived EVs isolated by ultracentrifugation (pellet) from pooled iPSC culture media (supernatant). (C) Representative TEM microphotographs of EVs isolated by differential centrifugation from culture media of human iPSCs. Original magnification, ×12–25,000. (D) Curve graph of DLS performed to determine the size of EVs isolated from iPSC-conditioned media. The majority of EVs range between 150 and 1000 nm in diameter. (E) Density (g/mL) of fractions collected after isolation of iPSC-EVs by density gradient centrifugation. (F) Representative immunoblots for purified EV markers MMP-2, PSGL1, CD63, and CD81 and cell marker Calnexin. Values represent mean ± SD from at least 3 independent experiments. *P < 0.05; Kruskal-Wallis test with post hoc Mann-Whitney test and Bonferroni’s correction.