Figure 5. Glycine substitution at residue 451 results in no overt structural defects in desmoplakin.

(A) Schematic of desmoplakin showing the location of the mutated residue (R451G). The mutation occurs near the NH₂-terminus region, which is responsible for interacting with the armadillo proteins of the desmosome. (B) Overlaid structural models of WT desmoplakin and desmoplakin with the R451G mutation after a 60-ns MD simulation. (C) Circular dichroism at 222 nm, normalized to 20°C for WT and R451G desmoplakin. As temperature was increased, both proteins showed similar degradation profiles.