Figure 2. Influenza HA targeting mAb FY1 inhibits viral replication and subsequent IFN production during influenza-associated bacterial pneumonia.

C57BL/6 mice infected with 100 PFUs of influenza A PR/8/34 and received a single dose of FY1 or IgG control on days 1 to 5 after influenza infection. Mice received secondary infection with 5 × 10⁷ CFUs of MRSA on day 6 after influenza and samples were harvested 24 hours following bacterial infection (n = 8). (A) Influenza matrix protein expression in lung tissue measured by RT-PCR (n = 8). (B–D) IFN gene expression in lung tissue measured by RT-PCR (n = 8). (E) IFN protein concentrations in lung tissue measured by Bio-plex (n = 8). *P < 0.05. Significance was tested by 1-way ANOVA using Dunnett’s multiple comparison test. Each experiment was independently performed twice, and data are shown from combined experiments. Data represent mean ± SEM.