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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Int J Dev Biol. 2015 Jan 1;59(7-9):313–325. doi: 10.1387/ijdb.150206am

Fig. 1. Effect of MO-based knockdown of TPC2 on the SMC-generated Ca2+ signals from 17 hpf to 30 hpf.

Fig. 1

Representative temporal profiles of the luminescence generated by α-actin-aeq transgenic embryos that were (Ai) untreated, or injected at the 1-4-cell stage with: (Bi) standard control-MO; (Ci) p53-MO; (Di) TPCN2-MOplus p53-MO; or (Ei) TPCN2-MO plus p53-MO, and TPCN2-mRNA at the amounts shown. (Dii, Eii) Temporal profiles of the luminescence when Triton X-100 was used at the end of each imaging experiment. (F-J) Histograms showing the mean ± SEM frequency of the Ca2+ signals generated every 30 min in the trunk musculature from 17 hpf to 30 hpf in embryos treated as described in panels A-E, respectively. Calcium signaling periods 1 and 2 (SP1 and SP2), and the signaling quiet period (QP; Cheung et al., 2011) are shown.