Fig. 2. Effect of bafilomycin A1 and trans-ned-19 on the SMC-generated Ca²⁺ signals from 17 hpf to 30 hpf.

Representative temporal profiles of the luminescence generated by α-actin-aeq transgenic embryos that were treated with (Ai) DMSO; (Bi) bafilomycin A1; or (Ci) trans-ned-19 at the concentrations shown, after the SP1 Ca²⁺ signals were first observed. To optimize the exposure of the muscles to the drugs, a small portion of the tail was removed from embryos just prior to treatment. (Bii, Cii) Temporal profiles of the luminescence generated following the addition of Triton X-100. (D-F) Histograms showing the mean ± SEM frequency of the Ca²⁺ signals generated every 30 min in the trunk musculature from 17 hpf to 30 hpf in embryos treated as described in panels A-C, respectively. The time of the tail-cut and duration of drug incubation are shown by the dotted line and grey shading, respectively. SP1, SP2 and the QP (Cheung et al., 2011) for an untreated control embryo are also shown.