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. Author manuscript; available in PMC: 2020 Jan 31.
Published in final edited form as: Nat Genet. 2019 Jul 31;51(8):1283–1294. doi: 10.1038/s41588-019-0471-2

Figure 5. PGCs accumulate unrepaired DNA breaks.

Figure 5

a, Representative images of 53BP1 foci in PGCs (GOF18-GFP+) from embryos at E11.5 and quantification of the frequency of PGCs with > 5 53BP1 foci per nucleus (P value calculated by unpaired 2-tailed t test; data represent mean and s.e.m.; at least 100 PGCs were scored per embryo, each point represents data from one embryo, n = 5, 3 and 3, left to right). b, Representative images of γ-H2A.X foci in PGCs (GOF18-GFP+) at E11.5 and quantification of the frequency of PGCs with > 10 γ-H2A.X foci per nucleus (P value calculated by unpaired 2-tailed t test; data represent mean and s.e.m.; at least 100 PGCs were scored per embryo, each point represents data from one embryo, n = 4, 4 and 5, left to right). c, Single-cell electrophoresis of FACS-sorted PGCs (SSEA1+GOF18-GFP+) at E11.5 (P value calculated by unpaired 2-tailed t test; data represent mean and s.e.m.; at least 100 PGCs were scored per embryo, n = 3 embryos per genotype). d, Quantification of PGCs (GOF18-GFP+) with > 5 γ-H2A.X foci per nucleus in wildtype or Fanca-/- embryos during embryonic development (E9.5-12.5). (P value calculated by unpaired 2-tailed t test; data represent mean and s.e.m.; each point represents data from one embryo, n = 4, 5, 2, 2, 3, 2, 4 and 7, left to right). e, Quantification of PGCs (SSEA1+GOF18-GFP+) by flow cytometry in wildtype and Fanca-/- embryos during embryonic development (P value calculated by 2-tailed Mann-Whitney test; data represent mean and s.e.m.; each point represents data form one embryo, n = 13, 13, 8, 6, 9, 13, 14 and 28, left to right).