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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Physiol. 2019 Jul 3;597(15):3999–4012. doi: 10.1113/JP278119

Figure 9. Differential expression of myofilament protein isoforms in nuclear bag and chain fibers.

Figure 9

A. Immunofluorescence staining of isolated spindles from WT mouse soleus muscle revealed that cTnT and ssTnT were expressed in nuclear bag (NB) fibers (stained by mAb CT3) whereas fsTnT was expressed exclusively in nuclear chain (NC) fibers (stained by mAb T12). B. Immunohistochemistry staining showed that the expressions of cTnT/ssTnT and fsTnT were restricted to two of the four intrafusal fibers of WT mouse muscle spindles whereas only one of the two cTnT/ssTnT expressing NB fibers expresses cardiac MHC (upper pane). This pattern was retained in spindles of ssTnT-KO mice except that cTnT was expressed in the NB fibers in the absence of ssTnT (lower panel).