Table 2.

Primer sequences for RT‐qPCR analysis

Genes	Primer sequences
β‐actin	Forward: 5′‐TCATCACCATTGGCAATGAG‐3′
	Reverse: 5′‐CACTGTGTTGGCGTACAGGT‐3′
CD44	Forward: 5′‐ATGGGTTCATAGAAGGGCACG‐3
	Reverse: 5′‐TGTCATACTGGGAGGTGTTGGA‐3
PTEN	Forward: 5′‐CTCAGCCGTTACCTGTGTGTG‐3
	Reverse: 5′‐AGGTTTCCTCTGGTCCTGGTA‐3
AKT1	Forward: 5′‐ATTGTGAAGGAGGGTTGGCTG‐3
	Reverse: 5′‐CCGCTCCTTGTAGCCAATGAA‐3
AKT3	Forward: 5′‐GGAGAGGAAGAGATGGATGCCT‐3
	Reverse: 5′‐CCACTTGCCTTCTCTCGAACC‐3
mTOR	Forward: 5′‐CCCTCCATCCACCTCATCAGT‐3
	Reverse: 5′‐CGCCAAGACACAGTAGCGAAT‐3
Cyclin B1	Forward: 5′‐GGTTGTTGCAGGAGACCATGT‐3
	Reverse: 5′‐AACATGGCAGTGACACCAACC‐3
Cyclin D1	Forward: 5′‐TTCATTTCCAATCCGCCCTCC‐3
	Reverse: 5′‐TGTGAGGCGGTAGTAGGACAG‐3
MMP1	Forward: 5′‐CTCTGGAGTAATGTCACACCTCT‐3
	Reverse: 5′‐TGTTGGTCCACCTTTCATCTTC‐3
MMP9	Forward: 5′‐GGGACGCAGACATCGTCATC‐3
	Reverse: 5′‐TCGTCATCGTCGAAATGGGC‐3
Ve‐cad	Forward: 5′‐TGTTCACGCATCGGTTGTTCA‐3
	Reverse: 5′‐TACATGACAGAGGCGTGGTCT‐3

Abbreviations: RT‐qPCR, Reverse transcription‐quantitative polymerase chain reaction; Ve‐cad, vascular endothelial‐cadherin.