. 2019 Jun 18;8(9):4370–4379. doi: 10.1002/cam4.2314

Table 1.

Primers for detection of non‐H pylori Helicobacter

Target gene	Target species	Primer sequences (F: forward, R: reverse)	Annealing temperature (℃）	Cycle number	Fragment size (bp)
ureA	H suis	F: CACCACCCCGGGGAAGTGATCTTG	60	40	253
ureA	H suis	R: CTACATCAATCAAATGCACGGTTTTTTCTTCG	60	40	253
ureA	H bizzozeronii	F: CGCTTTGAACCCGGTGAGAAAA	60	40	172
ureA	H bizzozeronii	R: TATCGCAACCGCAATTCACAACA	60	40	172
ureB	H felis	F: TCCCACTACCGGGGATCGTG	60	40	350
ureB	H felis	R: CAGCGGTTACAATCAAGCCCTCA	60	40	350
ureAB	H salomonis	F: CTTTGGGTCTGTGCCTGCCTG	62	40	219
ureAB	H salomonis	R: CATCGCGGATAGTCTTACCGCCT	62	40	219
ureA	H heilmannii ss	F: CTTTCTCCTGGTGAAGTGATTCTC	60	40	368
ureA	H heilmannii ss	R: CAGTTGATGGTGCCAAAG	60	40	368