Figure 5.

Recombinant human calcineurin B subunit (rhCNB) enhances the proportions of CD8 and CD4 T cells via a Toll‐like receptor 4 (TLR4)‐dependent process. A, rhCNB increases CD3⁺CD8⁺ proportions in splenocytes in a dose‐dependent manner B, rhCNB increases CD3⁺CD8⁺ proportions in the splenocytes of wild‐type (WT) mice, but reduces them in those of TLR4^−/− mice. C, rhCNB up‐regulates CD3⁺CD4⁺ proportions in the splenocytes of WT mice, but decreases them in those of TLR4^−/− mice. Three groups of WT mice were administered 20, 40, 80 mg/kg rhCNB, respectively, by peritoneal injection over 2 weeks. TLR4^−/− mice received 40 mg/kg rhCNB by peritoneal injection over 2 weeks. Splenocytes were isolated from all mice and subjected to FACs analysis