Figure 2.

Expression of apoptosis regulation related genes in C26 tumor cells after mEHT treatment. Significant reduction in the anti‐apoptotic XIAP, BCL‐2, BCL‐XL mRNA levels 1 and 3 h posttreatment (A). Elevated pro‐apoptotic PUMA mRNA levels at 1, 3, and 9 h, and BAX levels at 1 and 9 h after mEHT (B). Similarly increased temporal pattern of P21 mRNA levels to that of PUMA (C). In line with the apoptosis‐promoting mRNA profile, cleaved caspase‐3 protein expression (arrowheads) was significantly elevated 24 h after treatment as tested with immunocytochemistry (D). Scale bar: 100 µm. Significantly reduced colony‐forming tumor progenitor‐cell populations 10 d after mEHT treatment (E). *P < 0.05; **P < 0.01; ***P < 0.001