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. 2019 Aug 2;10(8):576. doi: 10.1038/s41419-019-1814-8

Fig. 4. circHECTD1 binds directly to miR-1256 to facilitate GC progression.

Fig. 4

a Complementary sequence between circHECTD1 and miR-1256. b miR-1256 expression was examined in GC tissues and matched peritumor samples using qRT-PCR. The expression level of miR-1256 was significantly lower in GC tissues than in peritumor samples. The data are shown as the mean ± SEM, n = 30. ***P < 0.001 vs. peritumor tissues. c A significant negative correlation between circHECTD1 and miR-1256 was detected in GC tissues. n = 30, P = 0.019. d qRT-PCR was used to investigate the levels of miR-1256 in circHECTD1-silenced BGC823 cells and circHECTD1-overexpressing SGC7901 cells. The data are shown as the mean ± SEM, n = 3. ***P < 0.001 vs. NC. e An RNA immunoprecipitation (RIP) assay for AGO2 was performed to detect the levels of circHECTD1 and miR-1256 in AGO2 pellets. The data are shown as the mean ± SEM, n = 3. **P < 0.01, ***P < 0.001 vs. IgG. f Luciferase reporters containing wild-type (WT) or mutant (MUT) circHECTD1 transcripts were co-transfected with miR-1256 mimics or negative control. Luciferase activity was determined using the Dual-Luciferase Reporter System. The data are shown as the mean ± SEM, n = 3. **P < 0.01 vs. relative luciferase activity in the circHECTD1-WT with miR-control group