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. 2019 Aug 2;10(8):576. doi: 10.1038/s41419-019-1814-8

Fig. 5. circHECTD1 enhances GC progression via miR-1256.

Fig. 5

a The efficiency of the miR-1256 inhibitor was verified using qRT-PCR. The data are shown as the mean ± SEM, n = 3. **P < 0.01 vs. miR-NC. b CCK-8 assays were performed to evaluate the effects of miR-1256 knockdown on the proliferative ability of BGC823 sh-circHECTD1 cells. The data are shown as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01 vs. sh-NC. c Transwell assays were used to analyze the effects of miR-1256 knockdown on BGC823 sh-circHECTD1 cells. The data are shown as the mean ± SEM, n = 3. **P < 0.01 vs. sh-NC. d The levels of glutamine, glutamate, and α-KG were examined in BGC823 sh-circHECTD1 cells with miR-1256 knockdown. The data are shown as the mean ± SEM, n = 3. **P < 0.01 vs. sh-NC. e Western blotting was performed to detect the expression levels of ASCT2 and GLS1 in circHECTD1-silenced BGC823 cells with miR-1256 knockdown