Fig. 8. Fluoxetine protected astrocytes in vivo and ameliorated depressive-like behaviors via promoting autophagy.

a, b Immunofluorescence images and cell counts of hippocampal GFAP⁺ astrocytes in fluoxetine and 3-MA treated CMS mice. Scale bar: 500 μm. c, d Immunofluorescence images of astrocytic morphology and quantitative analysis of total branch numbers in each group. Scale bar: 20 μm, n = 6. e, f Immobility time of fluoxetine and 3-MA treated CMS mice in FST and TST, respectively. n = 12, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 vs. Con group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. CMS group^{, $}p < 0.05, ^$$p < 0.01 vs. CMS+FLX group