Table 1.
Effect of CFCSs on membrane permeability of C. sakazakii ATCC 29544
| Percentages (%, mean ± standard deviation) of bacterial cells | |||
|---|---|---|---|
| Damaged | Dead | Live | |
| 30 min | |||
| C. sakazakii ATCC 29544 plus | |||
| L. acidophilus ATCC 4356 crude CFCS | 23 (± 2.1) | 12 (± 0.5) | 65 (± 0.2) |
| L. acidophilus ATCC 4356 2.5 × CFCS | 31 (± 1.1) | 14 (± 0.2) | 55 (± 1.2) |
| L. bulgaricus LBGR1 crude CFCS | 15 (± 1.5) | 5 (± 0.3) | 80 (± 0.7) |
| L. bulgaricus LBGR1 2.5 × CFCS | 21 (± 0.5) | 8 (± 1.0) | 71 (± 2.0) |
| L. casei rhamnosus ATCC 7469 crude CFCS | 25 (± 1.1) | 20 (± 2.5) | 55 (± 0.7) |
| L. casei rhamnosus ATCC 7469 2.5 × CFCS | 30 (± 0.5) | 32 (± 1.5) | 38 (± 1.2) |
| L. paracasei B21060 crude CFCS | 19 (± 1.7) | 4 (± 0.5) | 77 (± 0.5) |
| L. paracasei B21060 2.5 × CFCS | 22 (± 2.5) | 4 (± 0.2) | 74 (± 0.3) |
| L. salivarius ATCC 11741 crude CFCS | 10 (± 1.4) | 3 (± 0.7) | 87 (± 0.5) |
| L. salivarius ATCC 11741 2.5 × CFCS | 15 (± 0.7) | 5 (± 0.5) | 80 (± 1.2) |
| C. sakazakii ATCC 29544 | 1 (± 0.3) | 2 (± 0.2) | 97 (± 0.5) |
| 1 h | |||
| C. sakazakii ATCC 29544 plus | |||
| L. acidophilus ATCC 4356 crude CFCS | 12 (± 0.5) | 45 (± 0.5) | 43 (± 0.2) |
| L. acidophilus ATCC 4356 2.5 × CFCS | 36 (± 0.5) | 61 (± 1.5) | 3 (± 0.1) |
| L. bulgaricus LBGR1 crude CFCS | 17 (± 0.8) | 11 (± 0.2) | 71 (± 0.5) |
| L. bulgaricus LBGR1 2.5 × CFCS | 39 (± 0.1) | 25 (± 0.5) | 36 (± 0.3) |
| L. casei rhamnosus ATCC 7469 crude CFCS | 19 (± 0.2) | 74 (± 0.4) | 7 (± 0.3) |
| L. casei rhamnosus ATCC 7469 2.5 × CFCS | 14 (± 0.5) | 81 (± 0.3) | 5 (± 0.5) |
| L. paracasei B21060 crude CFCS | 20 (± 0.4) | 10 (± 0.5) | 70 (± 1.5) |
| L. paracasei B21060 2.5 × CFCS | 17 (± 0.5) | 13 (± 0.7) | 70 (± 0.9) |
| L. salivarius ATCC 11741 crude CFCS | 10 (± 0.3) | 5 (± 0.5) | 85 (± 0.5) |
| L. salivarius ATCC 11741 2.5 × CFCS | 20 (± 0.2) | 12 (± 0.6) | 68 (± 0.7) |
| C. sakazakii ATCC 29544 | 2 (± 0.5) | 1 (± 0.2) | 97 (± 0.2) |
| 2 h | |||
| C. sakazakii ATCC 29544 plus | |||
| L. acidophilus ATCC 4356 crude CFCS | 10 (± 0.5) | 57 (± 0.3) | 33 (± 0.5) |
| L. acidophilus ATCC 4356 2.5 × CFCS | 18 (± 0.4) | 81 (± 0.5) | 1 (± 0.5) |
| L. bulgaricus LBGR1 crude CFCS | 20 (± 0.5) | 18 (± 0.2) | 62 (± 0.2) |
| L. bulgaricus LBGR1 2.5 × CFCS | 48 (± 0.7) | 21 (± 0.5) | 31 (± 0.2) |
| L. casei rhamnosus ATCC 7469 crude CFCS | 16 (± 1.2) | 83 (± 1.0) | 1 (± 0.5) |
| L. casei rhamnosus ATCC 7469 2.5 × CFCS | 4 (± 0.5) | 96 (± 0.4) | 0 |
| L. paracasei B21060 crude CFCS | 14 (± 0.4) | 22 (± 0.5) | 64 (± 1.0) |
| L. paracasei B21060 2.5 × CFCS | 20 (± 0.4) | 17 (± 0.5) | 63 (± 0.1) |
| L. salivarius ATCC 11741 crude CFCS | 10 (± 0.5) | 6 (± 0.3) | 84 (± 0.2) |
| L. salivarius ATCC 11741 2.5 × CFCS | 37 (± 0.1) | 14 (± 0.2) | 49 (± 0.5) |
| C. sakazakii ATCC 29544 | 2 (± 0.4) | 3 (± 0.5) | 95 (± 0.4) |
Data represent the percentages of damaged, dead and live cells after exposure for 30 min, 1 h and 2 h to several crude and 2.5 × CFCSs, as determined by FCM using the double staining SYBR Green I and PI