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Scheme for preparation of human adipose tissue-derived extracellular matrix (ECM), growth factors (GFs), and stem cells. Human adipose-derived ECM is collected from liposuction, decellularized, and then manufactured to the required form. The GFs are prepared from decellularized ECM using optimized extraction buffer, then collected through dialysis and concentration. The stem cells are isolated from stromal-vascular fraction (SVF) through physical and enzymatic process
