Table 1.

Human adipose-derived ECM

Category	Contents		References
Decellularization methods	Physical	Freezing, pressure, agitation, sonication and radiation	[13, 15–20]
	Chemical	Detergent, acid, and alkaline
	Biological	Enzymes
Adipose ECM composition	Collagens	Type I–VII	[10, 16, 21–26]
	Non-collagen	Laminin, fibronectin, elastin and GAGs	[27–37]
Confirmation of decellularization	DNA quantification with PCR, IHC	< 50 ng ds DNA/mg < 200 bp in length	[10, 16]
	H&E stain	DAPI, acridine orange	[14]
Mechanical property (lyophilized)	Pore size	20 and 200 μm	[15]
	Porosity	≒ 90%
	Tensile strength	≒ 0.2 MPa
	Young’s modulus	≒ 65 MPa
	Maximum elongation	≒ 65%
Possible 3D scaffolds types	Sheet, microsphere, square, film, hollow tube, bead, injectable gel		[12, 16, 45]