Fig. 2.

Transmission electron microscopy (TEM) and confocal microscopy analysis of prepared SP, BCSP, and (BMP2 or BCSP)-conjugated scaffolds. Green fluorescence indicates the presence of green fluorescence protein (GFP) fused with BMP2 protein. A, B SP appear black after negative staining by TEM. C, D BCSP appear as shadows after negative staining by TEM. Shadows indicate that BMP2 was uniformly conjugated on the surface of the SP. E–G GFP-conjugated BMP2 on the surface of the BCSPs by confocal microscopy. H–J Surface of the BMP2-conjugated scaffold by confocal microscopy. K–M Surface of the BCSP-conjugated scaffold by conforcal microscopy