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. 2019 Jul 26;10:784. doi: 10.3389/fphar.2019.00784

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Copyright © 2019 Kawiak, Domachowska, Krolicka, Smolarska and Lojkowska

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of 3-chloroplumbagin on the expression levels of Bcl-2 family proteins and cytochrome c in breast cancer cells. MDA-MB-468 and MCF-7 cells were treated with the indicated concentrations of ChPL for 24 h, and the levels of Mcl-1, Bax, Bak, Bcl-2 and cytochrome c were assessed by Western blot analysis. Densitometric analysis represents protein levels normalized to β-actin levels or HSP60 levels. Data were analyzed by one-way ANOVA with Tukey’s post hoc tests [p < 0.01 (**), p < 0.001 (***), n = 3].