Table 1.
Primer pairs used for qPCR analysis
| Gene | Accession number | Primer sequence (5’ to 3’) | Product size (bp) | Reference |
|---|---|---|---|---|
| VP | M25646 | Forward: GCTGCTGCTTAGGCTGGTACA
Reverse: GGCGATGGCTCAGTAGACCC |
82 | Primer3 |
| GluA1 | NM_031608 | Forward: GTGTCTTCTCCTTTCTTGACCCTTT
Reverse: CTCTTCGCTGTGCCATTCGTAG |
128 | Primer3 |
| GluA2 | NM_017261 | Forward: AGAGAAAGAATACCCTGGAGCACA
Reverse: TCATCACTTGGACAGCATCATACG |
80 | Primer3 |
| GluA3 | NM_032990 | Forward: ATTCAGCAAATAGACCCATCTTAGC
Reverse: ATCACCACCAACAGGAAATACCAAA |
148 | Primer3 |
| GluA4 | NM_017263 | Forward: AAACAGGCAAAGATAGGAGAAAGGG
Reverse: TCAGAGAGGCATTGAAGACGATGG |
129 | Primer3 |
| GAPDH | X02231 | Forward: ACATGCCGCCTGGAGAAACCT
Reverse: GCCCAGGATGCCCTTTAGTGG |
90 | Kindlundh-Högberg et al. (2008) |
| Cytochrome C-1 | NM_001277194 | Forward: CTCTCCTCCTTGGACCACAC
Reverse: CGGTAAGCCACATAATCCA |
95 | Dachtler et al. (2014)* |
| Cyclophilin | M19533 | Forward: GAGCGTTTTGGGTCCAGGAAT
Reverse: AATGCCCGCAAGTCAAAGAAA |
90 | Kindlundh-Högberg et al. (2008) |
*
Adapted for rat.