FIG 2.

EBNA2 is important for MYC 525ESE1 activity. (A) Lentiviruses expressing 525ESE1-driven reporter were used to infect the GM12878 LCL and the P3HR1 Burkitt's lymphoma cells, which harbor mutant EBV with EBNA2 and the last two exons of EBNALP deleted. GFP expression levels were determined by FACS where the control was set to 1. NS, not significant. **, P < 0.01; *, P < 0.05; NS, not significant. (B) Luciferase reporter under the control of 525ESE1 was electroporated into BJAB cells in the presence or absence of EBNA2 expression plasmids. The empty expression plasmid control was set to 1. Renilla luciferase was used to normalize the transfection efficiency. EBNA2 expression levels were determined by Western blotting. **, P < 0.01; NS, not significant.