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. 2019 Jul 30;93(16):e00370-19. doi: 10.1128/JVI.00370-19

FIG 8.

FIG 8

SHFV nsp1β binds to poly(rC) binding protein 1 (PCBP1) and PCBP2. (A and C) Ectopically expressed SHFV nsp1β interacts with PCBP1 and PCBP2 in HEK-293T cells. HEK-293T cells were transfected with a plasmid expressing FLAG-tagged SHFV nsp1β or empty vector. The interaction between nsp1β and PCBP1/2 was determined by immunoprecipitation (IP) using anti-FLAG M2 antibody and Western blot (WB) analysis using antibodies against PCBP1/2. (B) SHFV nsp1β interacts with PCBP2 in virus-infected MARC-145 cells. MARC-145 cells were infected with SHFV at an MOI of 0.01 and harvested at 36 hpi. The interaction between nsp1β and PCBP2 was determined by immunoprecipitation (IP) with anti-PCBP2 mAb and Western blot (WB) analysis with mAb 76-69 against SHFV nsp1β. The expression of nsp1β and PCBP1/2 was monitored by WB analysis with specific antibodies. WCL, whole-cell lysate.