FIG 4.
B6-CD28KO mice did not display significant numbers of inflammatory cells compared to wild-type B6 mice. Corneas were removed from latently infected B6 (n = 10) and B6-CD28KO (n = 10) mice at day 17 following reactivation. These corneas were disaggregated into single-cell suspensions and stained with antibodies against CD45, CD4, CD8α, GR-1, and F4/80. The cells were then analyzed by flow cytometry. The data represent the mean ± SEM for individually analyzed corneas from these groups. Then, the number of each type of cell was significantly greater in wild-type B6 corneas. *, P values ranged from 0.01 to 0.005.