Characterization of sialic acid-mediated infection in iPSC motor neurons. Microfluidic chambers (n = 2 to 3 per experimental condition) containing iPSC motor neurons were treated with 100 mU/ml of neuraminidase on the axonal side for 1 h, followed by infection for 1 h with 1 × 105 TCID50 of EV-D68 IL/14-18952 (IL/52) (A), MO/14-18947 (MO/47) (B), CA/14-4231 (4231) (C), Fermon (D), Rhyne (E), or USA/N0051U5/2012 (US/TN) (F). Differences between the controls and the neuraminidase-treated groups were evaluated by Welch’s t test. ns, not significant.