FIG 4.

Sirtuin-modulating drugs impact MERS-CoV infection. (A) Huh7 cells were treated with the indicated concentrations of resveratrol at −2, 0, or +2 h relative to infection with MERS-CoV at an MOI of 0.1. Cells were incubated for 24 h and supernatant collected. Virus TCID₅₀ per milliliter in the supernatant was determined on Vero cells. Ethanol (EtOH) was the vehicle control for drug treatment. (B) As described for panel A, but cells were treated with EX527. For both panel A and panel B, data are from three independent experiments, with error bars representing standard deviations. (C) Cell viability was determined by CellTiter-Glo assay. Huh7 cells were treated with the indicated concentration of drug for 24 h prior to performance of the CellTiter-Glo assay to determine viability over a time period equivalent to that used in the MERS-CoV infection experiments. Error bars represent standard deviations of results of comparisons between 6 treatment wells from an experiment representing 3.