Antiviral activity of IFI6 isoforms. (A) Schematic representation of IFI6 isoforms. (B) Plasmids expressing FLAG-tagged IFI6 isoforms or FLAG-tagged controls were transfected into Huh7 cells. After 24 h, the cells were infected with ZIKV (MOI, 1). After 48 h, the cells were fixed and stained with flavivirus protein E and FLAG antibodies and analyzed using flow cytometry. The results are presented as a percentage of the FLAG control (TBC1D20-R105A). The results are the mean values ± SD. **, P < 0.01; ***, P < 0.001, one-way ANOVA with Tukey’s multiple-comparison test.