FIG 4.

Subcellular colocalization of HBZ and BRG1. Immunofluorescence was performed on HeLa cells that were transfected with an expression vector for either green florescent protein (GFP)-HBZ (A), MYC-BRG1 (B), or GFP-HBZ and MYC-BRG1 (C). At 24 h posttransfection, the cells were fixed and stained for BRG1 using anti-BRG1, followed by Alexa Fluor 594-conjugated goat anti-mouse IgG1 antibody (red). Nuclei were labeled with DAPI (blue), and slides were mounted using ProLong gold antifade reagent. Images were obtained using a Zeiss AxioImager MI fluorescence microscope and an AxioCam HR microscope camera.