FIG 8.

Cell cycle inhibitors have variable effects on BKPyV-induced G₂/M phase cell cycle arrest. (A) The cell cycle status of RPTE cells was determined under a number of different experimental conditions. RPTE cells were infected with BKPyV (MOI of 3) or mock infected and then subjected to CDK4/6 inhibition (1 μM PD0332991), CDK1/2 inhibition (20 μM Roscovitine), or CDK1 inhibition (5 μM RO-3306) at 24 hpi; cells were subsequently collected for analysis at 48 hpi. Collected cells were stained with propidium iodide (PI) and analyzed by flow cytometry (n = 3). Error bars represent standard deviations. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant (two-sample t test for changes in proportion of cells in G₁). (B) Histograms of PI staining for each experimental condition of a single experiment are shown. (C) Cell viability tests. RPTE cells were treated with 1 μM PD0332991, 20 μM Roscovitine, or 5 μM RO-3306 for 24 h and then subjected to a trypan blue exclusion assay according to the manufacturer’s protocol (n = 3).