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The EBNA1 mutants. (A) Coomassie gel of purified 6×His-SUMO tagged mutant proteins used in Biacore and EMSA studies. (B) Kd plots (fraction of complex versus [EBNA]) for all 6×His-SUMO-EBNA1 proteins binding to biotinylated DS4 DNA on the Biacore. Biacore sensorgrams used to make these plots are in Fig. S1. (C) Observed Kds for EBNA1s binding to DS4 were calculated by Biacore evaluation software. Biacore sensorgrams used to make these plots are in Fig. S1 (left).
