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Effects of PPRV N protein on IFN-β promoter activation induced by various adaptor molecules. HEK-293T cells were cotransfected with empty vector- or Flag-N-expressing plasmids and the indicated plasmids expressing RIG-I(CARD) (A), MDA5 (B), VISA (C), TBK1 (D), or IRF3 (E), together with IFN-β promoter-driven reporter plasmids. The luciferase activity was measured at 24 hpt. Expression of various adaptor molecules and PPRV N protein was evaluated by Western blotting.
