FIG 5.

hHB does not affect RIG-I or MDA5 expression. (A and B) Overexpression of hHB had no effects on the exogenous protein expression of RIG-I or MDA5. HEK293T cells were cotransfected with the indicated amounts of p3×Flag-hHB and pMyc-RIG-I or pMyc-MDA5 for 24 h, and RIG-I and MDA5 expression was analyzed by Western blotting, as indicated. (C and D) Overexpression of hHB did not affect RIG-I and MDA5 transcription. HEK293T cells were transfected with the indicated amounts of p3×Flag-hHB (hHB) for 24 h, and then RIG-I and MDA5 mRNA levels in cells were analyzed as indicated using real-time RT-PCR. (E) The mRNA levels of RIG-I and MDA5 exhibited no difference between the hHB^−/− and WT cells. The mRNA levels of RIG-I and MDA5 in the cells were analyzed using real-time RT-PCR. (F) The endogenous protein expression of RIG-I and MDA5 was unchanged in hHB overexpression cells. HEK293T cells were transfected with the indicated amounts of p3×Flag-hHB for 48 h, and the RIG-I and MDA5 expression levels were analyzed by Western blotting. (G) The endogenous protein expression of RIG-I and MDA5 in the hHB^−/− and WT cells was examined by Western blotting. NS, not significant (P > 0.05).