TABLE 1.
List of primers for real-time RT-PCR
| Primera | Sequence (5′–3′) |
|---|---|
| SeV N-F | AGTATGGGAGGACCACAGAATGG |
| SeV N-R | CCTTCACCAACACAATCCAGACC |
| EMCV 3D-F | TGAGCTTAGACCGATAGA |
| EMCV 3D-R | GATGCAAACTTTCCCAAC |
| GAPDH-F | GACACCCACTCCTCCACCTTT |
| GAPDH-R | ACCACCCTGTTGCTGTAGCC |
| IFN-β-F | CAAATTGCTCTCCTGTTGTGCTTC |
| IFN-β-R | AATGCGGCGTCCTCCTTCT |
| GBP1-F | TTCTTCCAGATGACCAGCAG |
| GBP1-R | GCTAGGGTGGTTGTCCTTGA |
| ISG56-F | GCCTTGCTGAAGTGTGGAGGAA |
| ISG56-R | ATCCAGGCGATAGGCAGAGATC |
| RIG-I-F | CACCTCAGTTGCTGATGAAGGC |
| RIG-I-R | GTCAGAAGGAAGCACTTGCTACC |
| MDA5-F | GCTGAAGTAGGAGTCAAAGCCC |
| MDA5-R | CCACTGTGGTAGCGATAAGCAG |
a
F: forward primer; R: reverse primer.