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. 2019 Jul 25;75(2):324–339.e11. doi: 10.1016/j.molcel.2019.05.008

Figure 4.

Figure 4

The NMD Cleavage Rate Is Variable and Depends on Exon Sequences Downstream of the PTC

(A) Schematic of indicated reporter.

(B–G) U2OS cells expressing scFv-sfGFP and PCP-mCherry-CAAX were transfected with indicated reporter plasmids (B–G) and UPF1 siRNA (D) and analyzed by time-lapse microscopy. The time from first detection of translation until mRNA cleavage was determined (B–F).

(B) Reporters were designed as in (A) and encoded an intron cassette of indicated genes.

(C) Cleavage rates for each reporter shown in (B).

(E) Schematic of indicated reporters.

(G) Quantification of the duration that individual mRNAs that were not cleaved could be observed over time.

Solid lines and corresponding shaded regions in (B), (D), (F), and (G) represent mean ± SEM. Dotted lines in (D) and (F) indicate that the data are replotted from an earlier figure panel for comparison. Error bars in (C) represent SEM. Number of measurements for each experiment are listed in Table S1. See also Figure S5.