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. 2019 Jul 25;75(2):324–339.e11. doi: 10.1016/j.molcel.2019.05.008

Figure 6.

Figure 6

The Number and Position of Introns Relative to the PTC Affects the NMD Decay Rate and Fraction NMD-Resistant mRNA Molecules

(A) Schematic of indicated reporters.

(B and E) U2OS cells expressing scFv-sfGFP and PCP-mCherry-CAAX were transfected with indicated reporter plasmids and analyzed by time-lapse microscopy.

(B) The time from first detection of translation until mRNA cleavage was determined.

(C and F) Genome-wide analysis of the effect of the number of downstream (C) or upstream (F) EJCs on NMD efficiency in a large cohort of cancer samples. Only PTCs with a single downstream EJC were included in (F).

(D) Schematic of indicated reporters.

(E) The time from first detection of translation until mRNA cleavage was determined.

Solid lines and corresponding shaded regions in (B) and (E) represent mean ± SEM. Dotted lines in (B) and (E) indicate that the data are replotted from an earlier figure panel for comparison. In boxplots of (C) and (F), the boxes represent the interquartile range with the central line depicting the median, and the whiskers extend to the extreme values after removing outliers. p values are indicated as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by two-tailed Mann-Whitney U tests. Number of measurements for each experiment are listed in Table S1. See also Figure S7.