Figure 7.

Acetylation-Mediated Control of Both J Head and Pds5 Chromatin Association

(A) Smc3 acetylation of heterodimers with E and J heads. Smc1-HA and Smc3 proteins containing hinge, E heads, J heads, or coiled coils cysteine pairs were cross-linked in vivo with BMOE. Complexes were immunoprecipitated on Scc1-PK, separated by SDS-PAGE, and analyzed by western blot against acetylated-Smc3 or non-acetylated Smc3. Percentage (mean ± SD of 3 independent experiments) of the cross-link signal is given for each antibody. ICC1(acetyl) = 0.79, ICC1(non-acetyl) = 0.72.

(B and C) Average calibrated ChIP-seq profiles of Scc1-PK (B) and Pds5-PK (C) in the presence or absence of Wpl1 and/or functional Eco1. Cells were arrested in G2 using nocodazole at restrictive temperature after release from pheromone arrest at permissive temperature.

(D) Averaged calibrated ChIP-seq profiles 60 kb either side of CDEIII plotted as a percentage of the average number of reads of Scc1-PK and Pds5-PK obtained for wpl1Δ cells in (B) and (C) respectively.

See also Figures S4, S5, and S6.