Figure 1.
Effect of AsiDNA and occurrence of resistance in KBM7 cells. Cells were treated with increasing doses of AsiDNA and assessed for (A) H2AX phosphorylation (γH2AX) and (B) PARP hyperactivation by measuring cellular PARylation 24 hours after treatment (n = 3), and (C) survival in the presence of AsiDNA 6 days after treatment (n = 3). The gray rectangle indicates the response dose range to AsiDNA, showing a correlation between cytotoxic effects and targets activation. Data are represented as mean ± SD. (D) Independent mutagenized KBM7 cell cultures (N) were treated for 2 weeks with AsiDNA (5 μM), olaparib (5 μM), imatinib (1 μM), or 6-TG (0.05 μg/ml). Doses were chosen to result in 5% survival (relative to nontreated cells); resistant cultures (N) were identified as those with high density (>5 105 cells/well) relative to the rest of the populations (frequencies of resistant clones are indicated as N/n).