Figure 3.

Gene expression profile after AsiDNA repeated treatments. MDA-MB-231 and NCI-H446 tumor cells or MCF-10A nontumor independent cell populations were treated with three cycles of AsiDNA (according to Figure 2A), tested for AsiDNA sensitivity before transcriptome analysis: (A) survival to AsiDNA of MDA-MB-231 (n = 8), NCI-H446 (n = 3), MCF-10A (n = 2). Data are mean ± SD. (B) transcriptional change 2 weeks after the third cycle of treatment. Hierarchical clustering of regulated genes. (C) Heatmap analysis showing the distance of AsiDNA three- cycle–treated (AsiDNA^3C) to mock-treated (NT) cell populations and Venn diagram representation with genes interacting with DNA-PK. Number of genes upregulated (red circle) or downregulated (green circle) in AsiDNA-treated populations is indicated. Distribution of genes interacting with DNA-PK according to KEGG classification in each group is indicated in the gray circle. Overrepresentation of DNA-PK interacting genes was calculated using a Student t test.