FIG 1.

16S rRNA gene phylogeny of Rummeliibacillus sp. strain TYF005. The 16S rRNA gene sequences of related taxa were obtained from GenBank, and multiple alignments were performed with the CLUSTAL W program (22). Phylogenetic analysis based on the 16S rRNA sequence was conducted using the maximum likelihood method based on the Kimura 2-parameter model (23) in MEGA7 (24) with 1,000 replications in a bootstrap test. The initial tree for the heuristic search was obtained automatically by applying the neighbor-join and BioNJ algorithms to a matrix of pairwise distances estimated using the maximum composite likelihood (MCL) approach and then selecting the topology with the superior log likelihood value. Bootstrap values of >50% are shown. Bacillus amyloliquefaciens strain MML2715, which comes from the next closest genus, was used as an outgroup.